Latest research on Calcitriol

Calcitriol or 1,25-dihydroxycholecalciferol (abbreviated 1,25-(OH)2-D3) is the active form of vitamin D found in the body (vitamin D3). Calcitriol is marketed under various trade names including Rocaltrol (Roche), Calcijex (Abbott) and Decostriol (Mibe, Jesalis). It is produced in the kidneys via 25-hydroxyvitamin D-1 α-hydroxylase by conversion from 25-hydroxycholecalciferol (calcidiol). This is stimulated by a decrease in serum calcium, phosphate (PO43−) and parathyroid hormone (PTH) levels. It regulates calcium levels by increasing the absorption of calcium and phosphate from the gastrointestinal tract, increasing calcium and phosphate reabsorption in the kidneys and inhibiting the release of PTH. Calcitriol is also commonly used as a medication in the treatment of hypocalcemia and osteoporosis.

Latest findings

Calcitriol (1, 25-dihydroxycholecalciferol), the hormonally active form of Vitamin D (Vit D), has long been known as an important regulator of Calcium homeostasis and bone metabolism through its activity in the intestines, bones, kidneys, and the parathyroid glands. [source, 2016]
Emerging evidence indicates that Calcitriol may be implicated in the regulation of other important biological processes such as insulin secretion, immune response, pro-differentiation, anti-proliferation, pro-apoptosis, anti-angiogenesis, inhibition of invasion and metastasis, and antiinflammation. [source, 2016]
It has recently been suggested that MIS constitutes a novel target regulated by Calcitriol in prostate cells, and that induction of MIS expression may play an important role in the anti-cancer activity of Calcitriol.1314 [source, 2016]
Growing evidence suggesting that MIS and Calcitriol can act synergistically to inhibit the growth of tumor cells prompted us to examine the effects of MIS in combination with Calcitriol on OCa cell lines. [source, 2016]
Therefore, the purpose of this study was to investigate whether MIS in combination with Calcitriol modulates the proliferation and apoptosis of human OCa cell lines (SKOV3, OVCAR3, and OVCA433) and to identify the signaling pathway by which MIS and Calcitriol mediate proliferation and apoptosis. [source, 2016]
Cells were seeded in a 96-well flat-bottomed plate (2×103 cells in 100 µL per well), incubated overnight to allow for cell attachment and recovery, and then exposed to MIS (USCN Life Science Inc., Wuhan, Hubei, China) and Calcitriol (Sigma, St. Louis, MO, USA) dissolved in methanol for 24, 48, 72, and 96 h. [source, 2016]
Cells were treated with Calcitriol for 4 h and then incubated with MIS for an additional 96 h. [source, 2016]
OCa cells were treated with a range of MIS (35.5-284 nM) and Calcitriol concentrations (1-100 µM) to determine the IC50 for each cell line. [source, 2016]
For all subsequent experiments, MIS and Calcitriol concentrations were held constant at or near their IC50, 71 nM and 50 µM, respectively (Fig. 1). [source, 2016]
SKOV3, OVCAR3, and OVCA433 cells were grown in plates for 48 h, and then treated with 71 nM MIS and 50 µM Calcitriol. [source, 2016]