Latest research on DMSO

A highly polar organic liquid, that is used widely as a chemical solvent. Because of its ability to penetrate biological membranes, it is used as a vehicle for topical application of pharmaceuticals. It is also used to protect tissue during cryopreservation. Dimethyl sulfoxide shows a range of pharmacological activity including analgesia and anti-inflammation. [PubChem]

Latest findings

Levels of cleaved PARP were calculated relative to DMSO control treated cells. [source, 2015]
For telaprevir (VX-950, peptidomimetic PI) resistance analysis, 48 h later, the culture medium was replaced with DMEM + FBS containing DMSO as a control or the indicated concentrations of telaprevir (5mM stock in DMSO, Selleckchem, Houston, TX, USA). [source, 2016]
The 10 mM AG490 liquid in DMSO was purchased from Sigma, and the same volume of DMSO was used as a vehicle control. [source, 2016]
To knock-down Csk, VSMCs were transiently transfected with control or Csk siRNA using Lipofectamine 2000 and treated for 24 hours with DMSO or PP2 after 24 hours transfection and then proteins were extracted for Western blotting. [source, 2016]
A pure culture of P. piscicida was isolated from open ocean plastic debris in the North Atlantic (Mincer culture ID; A757; Whalen et al., 2015), cryopreserved in 10% sterile DMSO, and stored at -85°C prior to experiments. [source, 2016]
Dimethyl sulfoxide (DMSO), pyrimethamine, and Sunitinib malate (SU 11248) were purchased from Sigma Aldrich (St. Louis, Missouri, USA). [source, 2016]
On the 5th day, the conditioning day, the animals were injected bilaterally with 1 μl of Actinomycin D (20 ng/μl) dissolved in 0.2 % Dimethyl sulfoxide (DMSO) or vehicle (0.2 % DMSO) into the GC. [source, 2016]
Samples were filtered (40 mm), washed with PBS, homogenized, isolated on a Lymphoprep gradient (Axis-Shield) and aliquoted in FCS plus 10% DMSO (Sigma-Aldrich) and stored in Liquid Nitrogen until required. [source, 2016]
On the 5th day, the saccharin pre-exposure day, the animals were injected into the GC bilaterally with 1 μl of Actinomycin D dissolved in 0.2 % Dimethyl sulfoxide (DMSO) or vehicle as a control (0.2 % DMSO). [source, 2016]
Then, cells were lysed with lysis buffer (10 mmol/L Tris, pH 10, containing 2.5 mmol/L NaCl, 100 mmol/L EDTA, 1% Triton X-100, and 10% DMSO, all from Sigma-Aldrich, Saint Louis, MO, USA) for 24 h at 4°C and in the dark. [source, 2016]