Latest research on EDTA

A chelating agent (chelating agents) that sequesters a variety of polyvalent cations. It is used in pharmaceutical manufacturing and as a food additive. [PubChem]

Latest findings

The nuclei was pelleted and incubated with 400 μl of SDS lysis buffer (1% SDS, 10 mM EDTA, and 50 mM Tris-HCl {pH 8.1}) containing proteinase inhibitor (Complete Mini, Roche) on ice for 10 min. [source, 2007]
Cell lysis was carried out in homogenization buffer (20 mM Tris-HCl, 5 mM DTT, 2 mM EDTA, 5 mM EGTA, protease inhibitor, phosphatase inhibitors, pH 7.5) with brief soni-cation (5 times for 10 sec each at 10% amplitude) after incubating on ice for 15-30 min. [source, 2016]
Labeled oligonucleotides (10,000 cpm), 10 µg of nuclear extracts and binding buffer [10 mM Tris-HCl, pH 7.6, 500 mM KCl, 10 mM EDTA, 50% v/v glycerol, 100 ng poly (dI·dC) and 1 mM dithiothreitol] were then incubated for 30 min at room temperature in a final volume of 20 µl. [source, 2016]
The beads were washed at least five times with IPP150 buffer followed by two washes with TE buffer (10 mM Tris HCl pH 8.0, 1 mM EDTA). [source, 2016]
To determine the expression of matrix metallopeptidase 9 (MMP9), insulin-like growth factor binding protein 3 (IGFBP3), CD14 and CD209 in the testis cells, antigen was retrieved by boiling for 30 min in tris-ethylenediamine tetra Acetic Acid (EDTA) solution. [source, 2016]
The chemical modulators (ethylene diamine tetra-acetic acid (EDTA), urea, PMSF, iodine, orlistat, and oleic acid) were set at 5 mM and after preincubation, BSL activity was determined under assay conditions. [source, 2016]
DNA from agarose beads was eluted by incubation with 150 μl elution buffer (50 mM Tris HCl pH 8.0, 10 mM EDTA, 1% SDS) at 65°C for 15 min. [source, 2016]
For IL-37 immunostaining, a microwave-based antigen retrieval process was employed with EDTA buffer, pH8.0, for 30 min. [source, 2016]
COS-7 cells were treated with or without EGF and then lysed into BOS buffer (50 mM Tris-HCl, pH 7.4, 200 mM NaCl, 1% Nonidet P-40, 10% glycerol, 10 mM NaF, 2.5 mM MgCl2, and 1 mM EDTA) with protease inhibitors. [source, 2016]
Approximately 2 μg of GST fusion proteins or 5 μg of purified His-tagged RhoA, Rac1, and Cdc42 proteins were incubated with 0.1 μg of active ERK1 protein in kinase buffer (5 mM MOPS, pH 7.2, 2.5 mM β-glycerophosphate, 5 mM MgCl2, 1 mM EGTA, 0.4 mM EDTA, 0.05 mM dithiothreitol) in the presence of 200 μM ATP and 5 μCi of [γ-32P]ATP at 30°C for 60 min in a volume of 25 μl. [source, 2016]