Latest research on Erbitux

Epidermal growth factor receptor binding FAB. Cetuximab is composed of the Fv (variable; antigen-binding) regions of the 225 murine EGFr monoclonal antibody specific for the N-terminal portion of human EGFr with human IgG1 heavy and kappa light chain constant (framework) regions.

Latest findings

Cells were seeded into 96-well tissue culture plates at the concentration of 2,000 cells/well and incubated at different concentrations of Cetuximab (Erbitux, Bristol Myers Squibb, NJ) (0.001–100 μg/ml), Cetuximab (1 nM–100 uM) or in combination with Ixabepilone (Ixampra, Bristol Myers Squibb, NJ) for 72 h. [source, 2016]
Currently, the standard of care (category 1 evidence) for recurrent/metastatic SCCHN is the regimen used in the EXTREME study (Erbitux in First-Line Treatment of Recurrent or Metastatic Head and Neck Cancer), consisting of cetuximab in combination with Cisplatin or Carboplatin plus 5-Fluorouracil (5-FU) [2, 3]. [source, 2016]
Cetuximab (Erbitux), trastuzumab (Herceptin), and panitumumab (Vectibix) were purchased from Merck (Whitehouse Station, NJ), Roche (Basel, Switzerland), and Amgen (Thousand Oaks, CA), respectively. [source, 2016]
The conjugation efficiency between bispecific antibody (Bis), Herceptin (Her), Erbitux (Erb), and both Herceptin and Erbitux (Erb+Her) with MnMEIO-CyTE777-mPEG NPs was determined by collecting unconjugated antibodies in the supernatant after centrifugal filtration of the antibody reaction mixture and measuring the concentration using NanoDrop ND-1000 UV-vis spectrometer. [source, 2016]
The variable domains of heavy (VH) and light (VL) chains of anti-EGFR (Erbitux) and anti-HER2/neu (Herceptin) were linked via (Gly4Ser)3 to form scFv (Erbitux) and scFv (Herceptin), respectively. [source, 2016]
The bispecific fragment was then generated by linking scFv (Erbitux) and scFv (Herceptin) with a flexible peptide linker, ASTGS, and cloned into the 5' region of the human IgG1 Fc in pFUSE-hIgG1e4-Fc vector to form an Fc-fusion construct (Fig. 1A). [source, 2016]
Tumor cells pretreated with excess Herceptin, Erbitux, and both antibodies, respectively, for 1 hr at 37 °C, followed by incubation with MnMEIO-CyTE777-(Bis)-mPEG NPs. [source, 2016]
Similarly, compared to non-pretreated group, 56.9% decrease was observed when A431 tumors were pretreated with Erbitux. [source, 2016]
There were 22.5% and 44.9%, respectively, decrease when tumor cells pretreated with Erbitux. [source, 2016]
Cetuximab (Erbitux, C225), monoclonal antibody to epidermal growth factor receptor (EGFR) is used as one of anti-EGFR agents for the treatment of metastatic colon and lung cancer. [source, 2016]