Latest research on Glutathione

A tripeptide with many roles in cells. It conjugates to drugs to make them more soluble for excretion, is a cofactor for some enzymes, is involved in protein disulfide bond rearrangement and reduces peroxides. [PubChem]

Latest findings

U0126, Y-27632, Glutathione cross-linked to 4% agarose, goat anti-mouse IgG conjugated with agarose, protein A conjugated with agarose, and Amino Amido Black staining solution were purchased from Sigma-Aldrich (St. Louis, MO). [source, 2016]
In addition, the antioxidant enzymes, especially Glutathione peroxidase-1 (GPX1), were upregulated in in vitro cultured pSSCs. [source, 2016]
GST-RhoA, GST-RhoA S88A, GST-RhoA T100A, GST-RhoA S88A/T100A, GST-RhoA S88E, GST-RhoA T100E, and GST-RhoA S88E/T100E purified proteins were eluted from the glutathione-sepharose beads using glutathione elution buffer (10 mM Reduced glutathione and 50 mM Tris-HCl, pH 8.0). [source, 2016]
Solution A consisted of hydroxylamine hydrochloride (0.3475 g), reduced Glutathione (0.1537 g), and N-CBZ-Gln-Gly (0.5060 g) dissolved in 0.2 mol/L Tris–HCl buffer (pH 6.0, 50 mL). [source, 2016]
Likewise, a large number of transcripts involved in ion homeostasis (cellular anion/cation homeostasis), such as those encoding for putative Glutathione S-transferases, amino acid permeases, phosphatidylinositol-4-phosphate 5-kinases and G3BP-like proteins, were also found to be differentially expressed in different chickpea cultivars and/or stress conditions (Supplementary Fig. S2b). [source, 2016]
Enzyme immunoassay of Glutathione (GSH) in serum was performed by using Six C USA Sandwich ELISA kit. [source, 2016]
Glutathione ELISA kit was procured from Tecan Group Ltd. [source, 2016]
Because the gut Mucosa contain relatively small amounts of antioxidant enzymes such as superoxide dismutase (SOD), catalase, Glutathione (GSH) peroxidase, it is possible that it may be overwhelmed during times of active inflammation which could result in intestinal injury [54]. [source, 2016]
Determinants of host immune response variability have been extensively reviewed by Datta De36 and mainly consist in variation in an inflammatory gene like IL-1 (RN 2/2 genotype), Glutathione S-transferase T1 null phenotype54 and a negative association with HLA-B35.55 [source, 2016]
Protease cleavage was stopped with 1 mM PMSF, and the solution containing GluA2 C-term was subsequently incubated with Glutathione Sepharose 4B-coupled GST-fusion proteins (GST and GST C-term from Myo9a). [source, 2016]