Latest research on Levothyroxine

The major hormone derived from the thyroid gland. Thyroxine is synthesized via the iodination of tyrosines (monoiodotyrosine) and the coupling of iodotyrosines (diiodotyrosine) in the thyroglobulin. Thyroxine is released from thyroglobulin by proteolysis and secreted into the blood. Thyroxine is peripherally deiodinated to form triiodothyronine which exerts a broad spectrum of stimulatory effects on cell metabolism. [PubChem]

Latest findings

Other drug interactions of a different/unknown mechanism included Digoxin and Levothyroxine (1 patient each). [source, 2002]
HPT were maintained in 1:1 Dulbecco's Modified Eagle Media:Ham's F12 containing 25 mM HEPES, 2 mM L-Glutamine, 100 U/ml penicillin/streptomycin, 250 μg/L Amphotericin B, 1 μg/ml Hydrocortisone, 10 μg/ml insulin, 5.5 mg/ml transferrin, 6.7 μg/ml selenium, 0.2 gm/L ethanolamine, 6.5 ng/ml L-Thyroxine, 10 ng/ml epidermal growth factor, and 10% fetal bovine serum (FBS). [source, 2002]
After adding L-T4 or D-T4 or NaOH only to the cell suspension (3 × 106 cells/ml), the probes were incubated for different periods. [source, 2001]
After 24-h incubation, the cells were washed three times with inositol-free Williams E medium, containing 0.1% BSA, then preincubated in the presence of 20 mM LiCl2 for 15 min, and incubated for 0.25 min in the presence of 0.1 μM of L-T4. [source, 2001]
It was determined that L-T4 rapidly induces the biphasic DAG accumulation in liver slices and isolated hepatocytes. [source, 2001]
The data obtained provide evidence that L-T4 activates PLC and -D in sequential manner that leads to increasing DAG formation during sustained agonist stimulation. [source, 2001]
It was demonstrated that L-T4 induced a biphasic formation of DAG peaking at 15 sec and 1-2 min (Fig. 1A, B). [source, 2001]
It has also been shown that L-T4 stimulates inositol 1,4,5-trisphosphate (Ins(l,4,5)P3) formations in [3H]inositol pre-labeled hepatocytes. [source, 2001]
L-T4 - induced DAG formation in hepatocytes was concentration-dependent and highly specific as D-T4 failed to accumulate DAG (Fig. 2A). [source, 2001]
L-T4 stimulatory effect on Peth formation was specific and concentration-dependent (Fig. 2B). [source, 2001]