Latest research on Rituxan

Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids

Latest findings

With Rituxan, which is a chimeric mAb comprising human γ1 Fc plus mouse anti-CD20 Fab regions, both ADCC and complement-dependent cytotoxicity (CDC) have been demonstrated in vitro, although the main determinants of its clinical efficacy have not been defined. [source, 2001]
Clynes's data (confirming reports by Funakoshi [14]) show a significant FcγRIII-dependent host component of anti-CD20 (Rituxan) in the response of xenografts in athymic mice. [source, 2001]
These constructs, unlike Herceptin and Rituxan, are monomeric, which might be a disadvantage (because of their lower affinity), but a new class of bsAbs has been designed that recognises tumour cell EpCAM antigen and CD3, and has an Fc composed of a mouse γ2a heavy chain and a rat γ2b heavy chain: like human γ1, two very potent activators of FcR. mAb BiUII has been shown to activate T cells expressing CD3, monocytes and macrophages expressing FcγRIII, NK cells expressing RI, but not B cells expressing RII/CD32 [18]. [source, 2001]
In these experiments, rheumatoid synovium was grafted into SCID mice, the animals were treated with anti-CD20 monoclonal antibody (Rituxan), and transcription of cytokines in situ was quantified from the retrieved grafts [70]. [source, 2000]