Latest research on Uracil

A thiourea antithyroid agent. Propythiouracil inhibits the synthesis of thyroxine and inhibits the peripheral conversion of throxine to tri-iodothyronine. It is used in the treatment of hyperthyroidism. (From Martindale, The Extra Pharmacopeoia, 30th ed, p534)

Latest findings

Then 1-2 μg of isolated DNA was diluted in 20 μL of water and used for bisulfite treatment by Wizard DNA Clean-Up System (Promega) kit which converts unmethylated cytosine to Uracil and leaves methylated cytosine unaltered. [source, 2016]
After exclusion of women on thyroid-related medications (Levothyroxine n=18, Propylthiouracil n=3) and women with overt biochemical hypothyroidism (n=10) or hyperthyroidism (n=1) on the blood samples, data from 956 women were included in this study. [source, 2016]
YPD (1 % yeast extract, 2 % peptone, 2 % glucose) and minimal CM media lacking Uracil were as described [67]. [source, 2016]
M9 was supplemented with 200 μM Uracil and 0.1 % maltose was added as a carbon source, yielding a growth rate of 0.6 dbl/h. [source, 2016]
MacFarlane et al., 2008), with a decreased flux to Thymidine synthesis associated with increased Uracil incorporation into DNA and enhanced cancer risk in some models (Macfarlane et al. [source, 2016]
By contrast, modulation of MTHFD1 has been shown to lower SAM levels but decrease Uracil incorporation into DNA, suggesting a switch toward nucleotide synthesis (MacFarlane et al. [source, 2016]
After end-repair (Enzymatics), dA-tailing with Klenow 3′-5′ (Enzymatics) and adapter ligation (Quick T4 DNA Ligase, NEB), the dUTP-containing second-strand was digested by Uracil DNA glycosylase (Enzymatics). [source, 2016]
In addition, the Uracil analogue, 5-fluoroUracil was able to repress the biofilm formation, and virulence gene in P. aeruginosa and E. coli4647. [source, 2016]
Sodium bisulfite treatment was performed using the EpiTect Bisulfite kit (Qiagen, Toronto, ON, Canada) to convert unmethylated cytosine residues to Uracil according to the supplier’s instructions. [source, 2016]
Supplementation of 1 mM Uracil or orotate during infection of HeLa cells restored the cytotoxicity of the pyrD mutant (Fig. 3a). [source, 2016]