Latest research on anti-RSV

Humanized monoclonal antibody (IgG1k) produced by recombinant DNA technology, directed to an epitope in the A antigenic site of the F protein of respiratory syncytial virus (RSV). Synagis is a composite of human (95%) and murine (5%) antibody sequences. The human heavy chain sequence was derived from the constant domains of human IgG1 and the variable framework regions of the VH genes Cor (1) and Cess (2). The human lightchain sequence was derived from the constant domain of Ck and the variable framework regions of the VL gene K104 withJk-4. Palivizumab is expressed from a stable murine (mouse) myeloma cell line (NS0). Palivizumab is composed of to heavy chains (50.6 kDa each) and two light chains (27.6 kDa each), contains 1-2% carbohydrate by weight and has a molecular weight of 147.7 kDa +/- 1 kDa (MALDI-TOF)

Latest findings

Maintenance of serum anti-RSV immunoglobulin concentrations during the RSV season is necessary for decreasing the risk of severe RSV disease [21]. [source, 2016]
Other agents currently in clinical trials include second-generation (more potent and half-life extended) mAb against F-protein, MEDI-8897, (MedImmune); REGN-2222, another anti-RSV F mAb, (Regeneron, Tarrytown, NY, USA); an inhaled nanobody from Ablynx (Ghent/Zwijnaarde, Belgium), ALX-0171, directed against F-protein; and the inhaled siRNA from Alnylam (Cambridge, MA, USA), ALN-RSV01, that targets a conserved epitope on N-protein. [source, 2016]
In a parallel analysis HEp2 cell monolayers were infected with RSV and between 1 and 4 dpi the monolayers were stained using anti-RSV and anti-mouse IgG-FITC (to detect virus-infected cells). [source, 2016]
This would be expected to give rise to a larger number of smaller and randomly distributed anti-RSV stained cell within the monolayer; rather than the formation of localized clusters of infected cells that we observe. [source, 2016]
Since infected cell clusters in the anti-RSV stained monolayer could be detected by 2 dpi, in a similar analysis we examined the distribution of individual virus structural proteins. [source, 2016]
In all cases infected cell clusters were observed at 2 dpi (Additional file 1: Figure S1A) similar to that observed with the anti-RSV staining. [source, 2016]
Cell monolayers were infected with RSV and at 1, 2 and 3 dpi the cells were stained with anti-RSV and examined by IF microscopy. [source, 2016]
The cells were infected with RSV and at between 1 and 5 dpi the monolayers were stained using anti-RSV and examined using IF microscopy. [source, 2016]
At 18 hpi the inhibitor was either maintained or removed and the cell stained with anti-RSV at 30 hpi (Fig. 4e). [source, 2016]
At 48 hpi the cells were stained using anti-RSV and examined using IF microscopy. [source, 2016]